HPLC Separation of Uridine and Uracil
https://sielc.com/Application-HPLC-Separation-of-Uridine-and-UracilChromatogram
Description
Uracil is a naturally occurring pyrimidine derivative. Uracil is used for drug delivery and as a pharmaceutical. Uridine is a product of uracil and sugar ribose. Uridine is one of components of ribonucleic acid. Uracil and uridine are slightly basic in nature. Both compounds are hydrophilic and uracil is very often is used as a void marker in reversed-phase chromatography. Uracil and uridine are separated based on their polar properties on a Primesep N HILIC column. Uracil and uridine are monitored by UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 90/10% |
|---|---|
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Class of Compounds | Nucleosides, Hydrophilic, Ionizable |
| Analyzing Compounds | Uracil, Uridine |
HPLC Column Used
Primesep N, 4.6×150 mm, 5 µm, 100A