HPLC Separation of Triadimefon on Obelisc and Primesep Columns
https://sielc.com/Application-HPLC-Separation-of-Triadimefon-on-Obelisc-and-Primesep-ColumnsChromatogram
Description
Triadimefon is a fungicide made up of a chlorophenol and a triazole. It is used on tropical fruit crops such as pineapple and banana. Primesep B2 and Obelisc R were used to retain triadimefon and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing triadimefon and dozens of other pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Triadimefon |
HPLC Column Used
Obelisc R, 2.1×150 mm, 5 µm, 100A