HPLC Separation of Succinylcholine and Choline Using a Primesep 200 Column
https://sielc.com/Application-HPLC-Separation-of-Succinylcholine-and-Choline-Using-a-Primesep-200-ColumnChromatogram
Method Parameters
| Mobile Phase | Gradient MeCN 0 – 20 %, 7 min |
|---|---|
| Buffer | TFA – 0.05-0.20% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 45C |
| Class of Compounds | Drug, Acid, Paralytic, Hydrophilic, Ionizable |
| Analyzing Compounds | Succinic Acid, Succinylcholine, Choline |
HPLC Column Used
Primesep 200, 4.6×50 mm, 5 µm, 100A