HPLC Separation of Salicylic acid
https://sielc.com/Application-HPLC-Separation-of-Salicylic-acidChromatogram
Description
Salicylic acid is a pain-reliever and anti-inflammatory commonly found in rubefacient products for topical pain relief as well as cosmetic skin-care products used to treat a variety of skin conditions. Legacy L1 was used to retain salicylic acid by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.
Method Parameters
| Mobile Phase | MeOH – 45% |
|---|---|
| Buffer | Acetic acid – 0.55% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Salicylic acid |
HPLC Column Used
Legacy L1, 4.6×250 mm, 5 µm, 100A