HPLC Separation of Polar Compounds
https://sielc.com/Application-HPLC-Separation-of-Polar-CompoundsChromatogram
Description
The separation of amino acids, the building blocks of proteins, can be challenging to separate on a reverse-phase column due to their high polarity. Using a mixed-mode HPLC column, allows the separation of amino acids by cation-exchange and ion-exclusion mechanisms as well as hydrophobicity. Fine tuning of separation can be achieved with changes in organic concentration of the mobile phase as well as choice of buffer and pH.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AcOH, AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Aspartic acid, Glutaric acid, Glycine, Hydroxytriptophan, GABA, Norepinephrine, Dopamine |
HPLC Column Used
Primesep 200, 4.6×150 mm, 5 µm, 100A