HPLC Separation of Phenylalanine, Glucosamine, and Tryptophan on Mixed-Mode Column
https://sielc.com/Application-HPLC-Separation-of-Phenylalanine-Glucosamine-and-Tryptophan-on-Mixed-Mode-ColumnChromatogram
Description
The Separation of Phenylalanine, Glucosamine and Tryptophan demonstrates how the small changes to the organic and/or buffer concentrations in the mobile phase affect the retention of compounds on a mixed-mode column by hydrophobic, ion-exchange and ion-exclusion mechanisms in reverse-phase HPLC chromatography. Evaporative Light Scattering Detector (ELSD) used
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm |
| Flow Rate | 0.25 ml/min |
| Detection | ELSD |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Phenylalanine, Glucosamine, Tryptophan |
HPLC Column Used
Primesep 100, 2.1×100 mm, 5 µm, 100A