HPLC Separation of Phenoxymethylpenicillin
https://sielc.com/Application-HPLC-Separation-of-PhenoxymethylpenicillinChromatogram
Description
Phenoxymethylpenicillin (also known as penicillin V) is an antibiotic useful in the treatment of multiple bacterial infections such as those caused by Streptococcus pyogenes, Anthrax, Lyme Disease, Rheumatic fever, and blood infection prophylaxis in children with sickle cell diseases. Legacy L1 was used to retain Phenoxymethylpenicillin by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.
Method Parameters
| Mobile Phase | MeCN – 30% |
|---|---|
| Buffer | NaH2PO4 pH 6.0 – 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Phenoxymethylpenicillin |
HPLC Column Used
Legacy L1, 4.6×250 mm, 5 µm, 100A