HPLC Separation of Organic Acids in HILIC and Anion-Exclusion Mode on Primesep S2 Column
https://sielc.com/Application-HPLC-Separation-of-Organic-Acids-in-HILIC-and-Anion-Exclusion-Mode-on-Primesep-S2-ColumnChromatogram
Description
Organic acids were separated on a HILIC/cation-exchange column in HILIC/anion-exclusion mode. This column can be used for analysis of polar compounds in HILIC mode. If compounds are ionizable, additional mode of interaction can be added (cation-exchange or anion-exclusion).
Method Parameters
| Mobile Phase | MeCN/H2O – 85/15% |
|---|---|
| Buffer | AmAc pH 5.0 15 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 50C UV 250 nm |
| Class of Compounds | Nucleosides, Hydrophilic, Ionizable |
| Analyzing Compounds | Toluenesulfonic acid, Naphthalenedisulfonic acid, 3.5 DHBA, Ascorbic acid, |
HPLC Column Used
Primesep S2, 4.6×150 mm, 5 µm, 100A