HPLC Separation of Organic Acids in HILIC Mode on Primesep N Column
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Description
Ascorbic, methylmalonic and succinic are weak organic acids. Retention of these three acids is achieved on Primesep N column in HILIC mode using acetonitrile/water and ammonium acetate. Compounds are monitored by ELSD. Method can be used for determination of ascorbic acid (Vitamin C), methylmalonic acid and succinic acid in various matrices. Other polar organic acids can be analyzed on this HILIC column.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Acid, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Ascorbic acid (Vitamin C), Methylmalonic acid, Succinic acid |
HPLC Column Used
Primesep N , 4.6×150 mm, 5 µm, 100A