HPLC Separation of NyQuil Active Ingredients
https://sielc.com/Application-HPLC-Separation-of-NyQuil-Active-IngredientsChromatogram
Description
NyQuil is a cough medication produced by Procter&Gamble. It consists of acetaminophen, pseudoephedrine, doxylamine and dextromethorphan. Most of the analytical HPLC methods include either use of ion-pairing reagent or a gradient elution. Mixed-mode chromatography allows to replace long gradient method with short isocratic method. All four compounds are well separated within 3-5 minutes. Methods can be optimized for analysis of other cough composition containing general cough suppressants and pain killers. Method is robust and being validated at several drug companies.
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | TEAPh |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Drug, Analgetic, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Acetaminophen, Pseudoephedrine, Doxylamine, Dextromethorphan |
HPLC Column Used
Primesep C, 4.6×50 mm, 5 µm, 100A