HPLC Separation of Nucleosides and Deoxynucleosides
https://sielc.com/Application-HPLC-Separation-of-Nucleosides-and-DeoxynucleosidesChromatogram
Method Parameters
| Mobile Phase | MeCN/MeOH |
|---|---|
| Buffer | AmFm, Formic acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Thymidine, Uridine, Deoxyadenosine, Adenosine, Deoxyguanosine, Guanosine, Deoxycytidine, Cytidine |
HPLC Column Used
Sharc 1, 4.6×150 mm, 5 µm, 100A