HPLC Separation of Nucleic Bases at pH 4 and 5 on Obelisc N
https://sielc.com/Application-HPLC-Separation-of-Nucleic-Bases-At-PH-4-and-5-on-Obelisc-NChromatogram
Description
Nucleic bases are biological compounds found in genetic molecules (DNA, RNA). They can be separated on an Obelisc N column, which offers very polar characteristics and can be used with positively or negatively charged groups. Closely-eluted adenosine and uridine can be further separated by simply adjusting the pH of the mobile phase. Mobile phase is water and acetonitrile (MeCN, ACN) with Ammonium Acetate as buffer. UV detection at 250nm.
Method Parameters
| Mobile Phase | MeCN -90% |
|---|---|
| Buffer | AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Uracil, Uridine, Adenosine, Guanosine, Cytidine, Cytosine |
HPLC Column Used
Obelisc N, 4.6×150 mm, 5 µm, 100A