HPLC Separation of Nabam on Mixed-Mode Columns
https://sielc.com/Application-HPLC-Separation-of-Nabam-on-Mixed-Mode-ColumnsChromatogram
Description
Nabam is a pesticide that controls algae, bacteria and fungal growth in aquatic industrial settings, and food processing water systems such as those used for cane and beet sugar production. Analysis of nabam is useful outside of agricultural science and food production since it can be used as a biocide for industrial purposes. Primesep 100 and Obelisc R were used to analyze nabam. Method is LC/MS compatible, and can be used for dozens of pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Nabam |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A