HPLC Separation of Metribuzin on Mixed-Mode HPLC Columns
https://sielc.com/Application-HPLC-Separation-of-Metribuzin-on-Mixed-Mode-HPLC-ColumnsChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Metribuzin. Metribuzin is a pre- and post- emergence herbicide on the EURL (European Union Reference Laboratory) Target Pesticide List for the EUPT-CF9 2015 (European Union Proficiency Test for Cereals and Feeding stuff). Metribuzin disrupts photosynthesis in plants growing near food crops such as potatoes, tomatoes and sugar came. Obelisc R and Primesep 100 were used to retain Metribuzin using multiple modes of separation. Obelisc R contains a long hydrophobic chain and ion-pairing groups, while Primesep 100 contains embedded acidic ion-pairing groups. Method is LC/MS compatible and can be used with similar conditions to retain many pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Metribuzin |