HPLC Separation of Isomers of Aminobutyric Acids
https://sielc.com/Application-HPLC-Separation-of-Isomers-of-Aminobutyric-AcidsChromatogram
Description
Primesep C separates the isomers of aminobutyric acids by a combination of reversed-phase and ionic interaction mechanisms. alpha-Aminobutyric acid, beta-Aminobutryic acid, and gamma-Aminobutyric acid (GABA) are baseline resolved without ion-pair reagents. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) ammonium acetate with evaporative light scattering detection (ELSD).
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmAc pH 4.1 |
| Flow Rate | 0.5 ml/min |
| Detection | ELSD |
| Class of Compounds | Acid |
| Analyzing Compounds | Alpha-Aminobutyric acid, Beta-Aminobutryic acid, Gamma-Aminobutyric acid (GABA) |
HPLC Column Used
Primesep C, 3.2×150 mm, 5 µm, 100A