HPLC Separation of Isomers of Amino Salicylic Acid
https://sielc.com/Application-HPLC-Separation-of-Isomers-of-Amino-Salicylic-AcidChromatogram
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | AmFm pH 3.0- 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 256 nm, MS-compatible mobile phase |
| Class of Compounds | Acids |
| Analyzing Compounds | 5-aminosalicylic acid, 4-aminosalicylic acid |
HPLC Column Used
Newcrom A, 4.6×150 mm, 5 µm, 100A