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Chromatogram

Description

Organic and inorganic acids and ions can be separated on a Primesep B4 column based on their ionic properties. Method can be used for quantitation of residual acids in various products and sample matrices. Trifluoracetic, hydrochloric, methanesulfonic, and nitric acids are separated using ACN-water-ammonium formate. Ions can be detected by ELSD, CAD or LC/MS.

Method Parameters

Mobile PhaseMeCN/H2O
BufferAmFm
Flow Rate1.0 ml/min
DetectionELSD
Class of CompoundsIons, Hydrophilic, Ionizable
Analyzing CompoundsSodium, Phosphate, Chloride,  Nitrate, Sulfate, Iodide, Perchlorate,Trifluoracetic

HPLC Column Used

Primesep B4, 4.6×150 mm, 5 µm, 100A