HPLC Separation of Guanidine and Benzoic Acids on Primesep 100 Column

https://sielc.com/Application-HPLC-Separation-of-Guanidine-and-Benzoic-Acids-on-Primesep-100-Column
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Chromatogram

Method Parameters

Mobile PhaseMeCN/H2O
BufferTFA
Flow Rate1.0 ml/min
DetectionUV, 230 nm, ELSD
Class of CompoundsAcid, Hydrophilic, Ionizable
Analyzing CompoundsGuanidine, Benzoic acid, Dinitrophenylacetic Acid

HPLC Column Used

Primesep 100, 4.6×150 mm, 5 µm, 100A
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