HPLC Separation of Glyphosate Reaction Intermediates and Impurities

https://sielc.com/Application-HPLC-Separation-of-Glyphosate-Reaction-Intermediates-and-Impurities
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Chromatogram

Description

Glyphosate and intermediates/impurities of production are separated on an Obelisc N HILIC/ion-exchange column by a combination of HILIC and ion-exchange mechanism. Method can be used in analysis of glyphosate, iminodiacetic acid, (N-phosphonomethyl)-imminodiacetic acid, diethanolamine and related impurities in reaction mixtures, waste and ground waters. Detection techniques are LC/MS, ELSD, CAD and UV.

Method Parameters

Mobile PhaseMeCN/H2O
BufferAmmonium Formate
Flow Rate1.0 ml/min
DetectionELSD
Class of CompoundsHerbicide, Hydrophilic, Ionizable
Analyzing CompoundsGlyphosate, IDA, PMIDA, DEA

HPLC Column Used

Obelisc N, 4.6×150 mm, 5 µm, 100A
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Data source & disclaimer: The HPLC application data, chromatograms and method parameters on this page are sourced from SIELC Technologies and are provided for informational and educational purposes only. All trademarks, column names and product designations remain the property of their respective owners.
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