HPLC Separation of Cyclodextrin Sulfobutyl Ether and Hydrophobic Compound
https://sielc.com/Application-HPLC-Separation-of-Cyclodextrin-Sulfobutyl-Ether-and-Hydrophobic-CompoundChromatogram
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Description
Cyclodextrin sulfobutyl ether is sulfonated derivative of cyclodextrin. The degree of sulfobutylation varies, and the compound is a mixture of several components. All compounds are very polar and acidic in nature, and are not retained by traditional reversed-phase chromatography. Separation was achieved on an Obelisc N column in anion-exchange mode. Hydrophobic naphthalene was used a a reference standard. Cyclodextrin was monitored by ELSD.
Method Parameters
| Mobile Phase | MeOH, THF |
|---|---|
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 60C UV 270 nm |
| Class of Compounds | Hydrophobic, Ionizable |
| Analyzing Compounds | Sodium, Beta Cyclodextrin Sulfobutyl Ether, Naphthalene |
HPLC Column Used
Obelisc N2, 4.6×150 mm, 5 µm, 100A