HPLC Separation of Creatine, Creatinine and Histidine with MS Conditions
https://sielc.com/Application-HPLC-Separation-of-Creatine-Creatinine-and-Histidine-With-MS-ConditionsChromatogram
Description
Creatine, creatinine, and histidine are all important amino acids in the human body. All of these can be separated by liquid chromatography. The mobile phase contains acetonitrile (MeCN), water, and phormic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | Formic Acid – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Creatine, Creatinine, Histidine |
HPLC Column Used
Primesep 200, 3.0×150 mm, 5 µm, 100A