HPLC Separation of Cotinine Metabolites
https://sielc.com/Application-HPLC-Separation-of-Cotinine-MetabolitesChromatogram
Description
Primesep 200 separates the very hydrophilic cotinine metabolites. Ion-exchange and other polar interactions provide retention for a compound that is difficult to retain on traditional C18 reversed-phase columns. The separation uses a simple mobile phase of water, acetonitrile (MeCN, ACN), and trifluoracetic acid (TFA) with UV detection.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 23, 250, 270 nm |
| Class of Compounds | Hydrophilic |
| Analyzing Compounds | Cotinine Metabolites |
HPLC Column Used
Primesep 200, 3.0×50 mm, 5 µm, 100A