HPLC Separation of Citrulline and Arginine on Primesep 200 Column
https://sielc.com/Application-HPLC-Separation-of-Citrulline-and-ArginineChromatogram
Description
Citrulline and Arginine are α-amino acids that differ only in one functional group. They can be separated on a reverse-phase Primesep 200 HPLC column by hydrophobic and cation-exchange mechanisms in a mobile phase of water and acetonitrile (MeCN, ACN) with a gradient buffer of formic acid. Evaporative Light Scattering Detector (ELSD) used.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | Formic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Citrulline, Arginine |
HPLC Column Used
Primesep 200, 4.6×50 mm, 5 µm, 100A