HPLC Method for Analysis of Cetylpyridinium Chloride and Triethylene Glycol on Obelisc N Column
https://sielc.com/Application-HPLC-Separation-of-Cetylpyridinium-Chloride-and-Triethylene-GlycolChromatogram
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Description
Cetylpyridinium chloride is hydrophobic basic compound and triethylene glycol is hydrophilic neutral compounds. Quantitative analysis of both compounds is problematic due to a different nature of these two analytes. Both compounds were analyzed on an Obelisc N column in HILIC/cation-exchange mode. Cetylpyridinium chloride is retained by cation-exchange mechanism, and triethylene glycol is retained by HILIC mechanism. Mixed-mode HILIC approach allows to retain compounds either based on multiple or single mechanisms interaction, thus providing a valuable approach for analysis. Cetylpyridinium chloride and triethylene glycol can be monitored by combination of UV and ELSD/CAD.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmAc ph 5.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250nm, ELSD |
| Class of Compounds | Surfactant, Hydrophobic, Ionizable |
| Analyzing Compounds | Triethylene glycol, Cetylpyridinium Chloride |
HPLC Column Used
Obelisc N, 4.6×150 mm, 5 µm, 100A