HPLC Separation of Captan on Obelisc R and Primesep 100 Columns
https://sielc.com/Application-HPLC-Separation-of-Captan-on-Obelisc-R-and-Primesep-100-ColumnsChromatogram
Description
Captan is a fungicide that is typically combined with other pesticides. It is in the class of fungicides called phthalimide, and is used on a number of vegetables and ornamental plants. Captan reduces infections on the surface of plant material improving their appearance. The EURL-SRM (European Union Reference Laboratory – Single Residue Methods) included captan in a list of pesticides difficult to analyze by traditional multiresidue methods. We separated and analyzed captan using two mixed-mode columns with different modes of separation. Obelisc R has a long hydrophobic chain and multiple ion-pairing groups, and Primesep 100 contains acidic ion-pairing groups. Method is LC/MS compatible and can be use on many different pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70% |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Pesticide, Hydrophobic, Ionizable |
| Analyzing Compounds | Captan |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A