HPLC Separation of Caffeine, Benzoic acid and Acetaminophen

https://sielc.com/Application-HPLC-Separation-of-Caffeine-Benzoic-acid-and-Acetaminophen
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Chromatogram

Method Parameters

Mobile PhaseMeOH – 28%
BufferAcetic Acid – 3%
Flow Rate1.5 ml/min
DetectionUV, 275 nm
Class of CompoundsDrug, Acid,  Hydrophilic, Hydrophobic, Ionizable
Analyzing CompoundsCaffeine, Benzoic acid, Acetaminophen

HPLC Column Used

Legacy L1, 4.6×250 mm, 5 µm, 100A
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Data source & disclaimer: The HPLC application data, chromatograms and method parameters on this page are sourced from SIELC Technologies and are provided for informational and educational purposes only. All trademarks, column names and product designations remain the property of their respective owners.
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