HPLC Separation of Aspartic Acid and Asparagine on Obelisc R Column
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Description
Two amino acids, aspartic acid and asparagine, are separated on an Obelisc R column by combination of reverse-phase and ion-exchange mechanism. Method can be used for analysis of these and other underivatized amino acids by HPLC with UV, ELSD, CAD and LC/MS detection.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm, AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Asparagine, Aspartic Acid |
HPLC Column Used
Obelisc R, 4.6×150 mm, 5 µm, 100A