HPLC Separation of Aromatic Boronic Acids on Primesep P
https://sielc.com/Application-HPLC-Separation-of-Aromatic-Boronic-AcidsChromatogram
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Description
Boronic acid is alkyl, or aryl substituted, boric acid. Boronic acid can form complexes with sugars, amines, and amino acids. Four aromatic boronic acids were separated on a Primesep P mixed-mode reversed-phase cation-exchange column. Primesep P column also has an aromatic fragment which provides pi-pi interaction. Compounds can be monitored by UV, ELSD, CAD and LC/MS. Various mobile phases can be employed for HPLC analysis of boronic acids.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm |
| Class of Compounds | Drug, Acid, Hydrophobic, Ionizable |
| Analyzing Compounds | Chlorohenyl-4-boronic acid, Naphtalene-1-boronic acid, Biphenyl-4-boronic acid, 4-(1-naphthyl)phenylboronic acid |
HPLC Column Used
Primesep P, 4.6×150 mm, 5 µm, 100A