HPLC Separation of Aminobiphenyls
https://sielc.com/Application-HPLC-Separation-of-AminobiphenylsChromatogram
Description
Aminobiphenyls consist of isomers that differ only in the location of a primary amine group on an aromatic ring. 2-, 3-, and 4-aminobiphenyl are separated on a Primesep D with good peak shape and a short retention time by a mixture of reversed-phase and ion-exclusion interactions. Peak order can be reversed for 2- and 3-aminobiphenyls by using ammonium formate buffer without pH adjustment. The HPLC separations use a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate buffer or acetic acid (HOAc) with UV detection at 250 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | Formic Acid, AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Hydrophobic, Ionizable |
| Analyzing Compounds | 2-Aminobiphenyl, 4 -Aminobiphenyl, 3 -Aminobiphenyl |
HPLC Column Used
Primesep D, 4.6×150 mm, 5 µm, 100A