HPLC Separation of Aminobiphenyl Isomers in Cation-Exchange Mode
https://sielc.com/Application-HPLC-Separation-of-Aminobiphenyl-Isomers-in-Cation-Exchange-ModeChromatogram
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Description
Two isomers of aminobiphenyl were successfully retained and separated in cation-exchange mode on a Primesep S2 column. Primesep S2 column is a HILIC/cation-exchange column that can be operated in HILIC, cation-exchange, and anion-exclusion modes. Method and column are compatible with ELSD/LC/MS and UV and can be used for routine analysis of polar molecules.
Method Parameters
| Mobile Phase | MeCN/H2O – 5/95% |
|---|---|
| Buffer | AmAc pH 5.0 15 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Hydrophobic, Ionizable |
| Analyzing Compounds | 2-Aminobiphenyl, 4 -Aminobiphenyl |
HPLC Column Used
Primesep S2, 4.6×150 mm, 5 µm, 100A