HPLC Separation of Amino Acids on Obelisc R Column
https://sielc.com/Application-HPLC-Separation-of-Amino-Acids-on-Obelisc-R-ColumnChromatogram
Description
Closely related compounds like amino acids can be separated on an Obelisc R column by various buffers depending on the amount of baseline separation required. By choosing different buffers, the separation between compounds can be adjusted based on application needs, especially those that require low organic concentration in the mobile phase. UV detection at 250nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 5/95% |
|---|---|
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Amino acids |
HPLC Column Used
Obelisc R, 4.6×250 mm, 5 µm, 100A