HPLC Separation of Amino Acids in HILIC and Cation-Exchange Modes
https://sielc.com/Application-HPLC-Separation-of-Amino-Acids-in-HILIC-and-Cation-Exchange-ModesChromatogram
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Description
· Amino acids can be retained and separated on a Primesep S2 column in HILIC/cation-exchange mode.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Dopa, Tyrosine, Phenylalanine |
HPLC Column Used
Primesep S2, 4.6×150 mm, 5 µm, 100A