HPLC Separation of Amino Acids, Bases, Acids, and Neutrals on Obelisc R
https://sielc.com/Application-HPLC-Separation-of-Amino-Acids-Bases-Acids-and-Neutrals-on-Obelisc-RChromatogram
Description
Separating basic, acidic and zwitterionic compounds in one run in reverse-phase HPLC can be very challenging. The methods might require the use of ion-pairing reagents and complex gradients that can make MS-compatibility difficult. Obelisc R column which has both positive and negative ion-pairs embedded in the stationary phase allows for fine tuning and separation of a wide range of compounds with different ionic properties. Acids, bases, amino acids and neutral compounds were separated isocratically in one run using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer. Can also be UV detected at 250nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 35/65% |
|---|---|
| Buffer | AmAc 10 mM pH 4.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Acid, Bases, Neutral, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Amino acids |
HPLC Column Used
Obelisc R, 4.6×250 mm, 5 µm, 100A