HPLC Separation of Active Compounds in Drug Formulation
https://sielc.com/Application-HPLC-Separation-of-Active-Compounds-in-Drug-FormulationChromatogram
HPLC Separation of Active Compounds in Drug Formulation
Description
An HPLC method for the separation of active drug compounds on a Primesep 200 column. The retention of compounds is achieved through reverse-phase, cation exchange and hydrophobic interactions. Benzoic acid, hyoscyamine sulfate and phenyl salicylate are baseline separated using simple mobile phases of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with a UV detector.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Benzoic acid, Hyoscyamine Sulfate, Phenylsalicylate) |
HPLC Column Used
Primesep 200, 3.0×50 mm, 5 µm, 100A