HPLC Separation of Acetaminophen, Phenylephrine, and Diphenhydramine
https://sielc.com/Application-HPLC-Separation-of-Acetaminophen-Phenylephrine-and-DiphenhydramineChromatogram
Description
Primesep C separates acetaminophen (paracetamol), phenylephrine, and diphenhydramine– common over-the-counter cold and flu medication ingredients such as Tylenol products—by either an acetonitrile or phosphoric acid gradient. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid with ultraviolet (UV) detection at 210 nm.
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | H3PO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Drug, Analgetic, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Acetaminophen, Pseudoephedrine, Diphenhydramine |
HPLC Column Used
Primesep C, 4.6×150 mm, 5 µm, 100A