HPLC Separation of 3-Nitrophthalic and 4-Nitrophthalic Acids
https://sielc.com/Application-HPLC-Separation-of-3-Nitrophthalic-and-4-Nitrophthalic-Acids-on-Primesep-D-HPLC-ColumnChromatogram
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275nm |
| Class of Compounds | Acid |
| Analyzing Compounds | 3-Nitrophthalic and 4-Nitrophthalic Acids |
HPLC Column Used
Newcrom BH, 3.2×50 mm, 3 µm, 100A