HPLC Separation of 2-Naphthoxyacetic acid
https://sielc.com/Application-HPLC-Separation-of-2-Naphthoxyacetic-acidChromatogram
Description
2-Naphthoxyacetic acid was included in a EURL (European Union Reference Laboratory) report for using QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) single residue methods. Primesep 100 & Obelisc R were used to separate 2-Naphthoxyacetic acid. Primesep 100 is a mixed-mode column which uses embedded ion-pairing groups and Obelisc can fine tune separations using both a long hydrophobic chain and ionic groups. Method is LC/MS compatible and can be used for many pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Synthetic plant hormone, Acid, Hydrophobic, Ionizable |
| Analyzing Compounds | 2-Naphthoxyacetic acid |
HPLC Column Used
Primesep 100, 2.1×150 mm, 3 µm, 100A