HPLC Separation of 1-Methylhistidine and 3-Methylhistidine on Primesep 200
https://sielc.com/Application-HPLC-Separation-of-1-Methylhistidine-and-3-Methylhistidine-on-Primesep-200Chromatogram
Description
Application Notes: Methylhistidines are polar amino acids with very similar structures. Methylhistidines are methylated derivative of histidine found in actin. N-methylhistidine is released into urine after the breakdown of actin and myosin. Urinary output of N-methylhistidine is a reliable index of the rate of myofibrillar protein breakdown in musculature. The method for separation of 1-methylhistidine and 3-methylhistidine on Primesep 200 column was developed with LC/MS compatible conditions. This method can be used for quantitation of methylhystidines in urine and other biofluids.
Application Columns: Primesep 200 Application compounds: 1-Methylhistidine, 3-Methylhistidine Detection technique: UV, LC/MS, ELSD/CAD
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | AmFm pH 3.0- 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Amino acid , Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | 1-Methylhistidine, 3-Methylhistidine |
HPLC Column Used
Primesep 200, 4.6×150 mm, 5 µm, 100A