HPLC Retention of Terbufos on Obelisc N Mixed-Mode Column
https://sielc.com/Application-HPLC-Retention-of-Terbufos-on-Obelisc-N-Mixed-Mode-ColumnChromatogram
Description
Terbufos is an extremely toxic organophosphate insecticide and nematacide. Obelisc R was used to retain terbufos and separate it from impurities. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing terbufos, other organophosphate insecticides, and dozens of other pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Terbufos |
HPLC Column Used
Obelisc R, 2.1×150 mm, 5 µm, 100A