HPLC Retention of Chlorpyrifos-methyl on Primesep 100 and Obelisc R Mixed-Mode Columns
https://sielc.com/Application-HPLC-Retention-of-Chlorpyrifos-methyl-on-Primesep-100-and-Obelisc-R-Mixed-Mode-ColumnsChromatogram
Description
Chlorpyrifos-methyl, an organophosphate pesticide is considered a target pesticide by the EURL (European Union Reference Laboratory) for the EUPT-FF9 2015 a proficiency test for the analysis of a over a hundred pesticides. This compound was separated on two different mixed-mode columns, Primesep 100 and Obelisc R. Primesep 100 is a reverse phase column that contains embedded acidic ion-pairing groups, while Obelisc R retains with long hydrophobic chains and multiple ionic pairing groups on the surface. Method is LC/MS compatible and can be replicated to analyze dozens of other pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Chlorpyrifos-methyl |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A