HPLC Method for Analysis of Vicodin (Acetaminophen & Hydrocodone)
https://sielc.com/HPLC-Method-Analysis-VicodinChromatogram
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Method Parameters
| Mobile Phase | Gradient MeCN – 5-50% |
|---|---|
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Analgetic, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Acetaminophen, Hydrocodone |
HPLC Column Used
Primesep 200, 3.2×100 mm, 5 µm, 100A