HPLC Method for Analysis of Tamsulosin
https://sielc.com/HPLC-Method-Analysis-TamsulosinChromatogram
Chr_1044
Description
Tamsulosin is chemically 5-{(2R)-2[{2-(2-ethoxyphenoxy)ethyl} amino]propyl}-2-methoxybenzene sulfonamide and a selective α1 adrenoceptor blocking agent. It is used to treat BPH (benign prostatic hyperplasia). The treatment is associated with a low rate of serum aminotransferase elevations. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Tamsulosin. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-50% |
|---|---|
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Tamsulosin |
HPLC Column Used
Primesep 200, 3.2×100 mm, 5 µm, 100A