HPLC Method for Analysis of Propranolol
https://sielc.com/HPLC-Method-Analysis-PropranololChromatogram
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Description
Propranolol is a synthetic, non-cardioselective beta-adrenergic antagonist that is used for the therapy of hypertension, cardiac arrhythmias, angina pectoris, hyperthyroidism, as well as after a heart attack to improve the chance of survival. Dizziness, lightheadedness, or tiredness may occur after the intake of the medication. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Propranolol. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Method Parameters
| Mobile Phase | MeCN – 40% |
|---|---|
| Buffer | H3PO4 – 0.1%, |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | Propranolol |
HPLC Column Used
Primesep 200, 3.2×100 mm, 5 µm, 100A