HPLC Method for Analysis of MDMA
https://sielc.com/HPLC-Method-Analysis-MDMAChromatogram
Chr_1042
Description
Methylenedioxymethamphetamine (MDMA), also known as ecstasy, is a synthetic drug that has both, a psychedelic and stimulant effects. It is predominantly a “club drug” and is commonly used at rave parties. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain MDMA. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-50% |
|---|---|
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Methylenedioxymethamphetamine (MDMA) |
HPLC Column Used
Primesep 200, 3.2×100 mm, 5 µm, 100A