HPLC Method for Analysis of MDA
https://sielc.com/HPLC-Method-Analysis-MDAChromatogram
Chr_1043
Description
MDMA is a part of the amphetamine family that is mainly used as a recreational drug. It has a psychedelic effect and is a serotonin-norepinephrine-dopamine releasing agent (SNDRA). Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain MDA. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-50% |
|---|---|
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | 3,4-Methylenedioxyamphetamine, MDA |
HPLC Column Used
Primesep 200, 3.2×100 mm, 5 µm, 100A