HPLC Method for Analysis of Indapamide
https://sielc.com/HPLC-Method-Analysis-IndapamideChromatogram
Chr_1047
Description
Indapamide is an indole derivative of chlorosulphonamide and a sulfamyl diuretic with antihypertensive activity. It exerts its diuretic effect by inhibiting reabsorption of sodium and chloride, primarily as a result of action on the cortical diluting segment of the renal distal tubule. Indapamide is also used in the treatment of hypertension. Primesep 200, a reverse phase column , contains embedded acidic ionizable groups and can retain Indapamide. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-50% |
|---|---|
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Indapamide |
HPLC Column Used
Primesep 200, 3.2×100 mm, 5 µm, 100A