HPLC Method for Analysis of Fenofibric Acid and Fenofibrate on Primesep 200 column
https://sielc.com/HPLC-Method-Analysis-FenofibrateChromatogram
Description
Fenofibrate is a synthetic Peroxisome Proliferator Receptor alpha (PPAR alpha) Agonist as well as an antilipemic agent. It is used to help lower “bad” cholesterol and fats (LDL, triglycerides) by increasing the natural enzyme that breaks down fats in the blood. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Propranolol. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Method Parameters
| Mobile Phase | MeCN – 45% |
|---|---|
| Buffer | H2SO4 – 0.05%, |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 286 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | Fenofibrate |
HPLC Column Used
Primesep 200, 3.2×100 mm, 5 µm, 100A