HPLC Method for Analysis of Cocaine
https://sielc.com/HPLC-Method-Analysis-CocaineChromatogram
Chr_1041
Description
Cocaine, also known as coke, is mostly used as a recreational drug. It is a strong stimulant. The drug’s most common appearance in powder form is a salt, usually cocaine hydrochloride. Due to Cocaine rapidly metabolizing in the body, so if it’s ingested/inhaled, its concentration in urine usually does not exceed 10% that of its principal metabolite, benzoylecgonine. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Cocaine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-50% |
|---|---|
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Cocaine |
HPLC Column Used
Primesep 200, 3.2×100 mm, 5 µm, 100A