HPLC Method for Analysis of B-Estadiol

https://sielc.com/HPLC-Method-Analysis-B-Estadiol
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Chromatogram

Chr_1045

Chr_1045

Method Parameters

Mobile PhaseGradient MeCN – 5-50%
BufferGradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold
Flow Rate0.5 ml/min
DetectionUV, 270 nm
Class of CompoundsDrug, Acid, Hydrophilic, Ionizable,
Analyzing CompoundsB-Estadiol

HPLC Column Used

Primesep 200, 3.2×100 mm, 5 µm, 100A
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