HPLC Method for Analysis of Acetaminophen and Oxycodone
https://sielc.com/HPLC-Method-Analysis-Acetaminophen-OxycodoneChromatogram
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Description
Acetaminophen is a p-aminophenol derivative with analgesic and antipyretic activities. It has weak anti-inflammatory properties, may cause liver, blood cell, and kidney damage. It is a nonprescription medication for mild-to-moderate pain and fever.
Oxycodone is semi-synthetic opioid type drug that is used to help relieve moderate to severe pain. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Acetaminophen and Oxycodone. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-50% |
|---|---|
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Analgetic, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Acetaminophen, Hydrocodone |
HPLC Column Used
Primesep 200, 3.2×100 mm, 5 µm, 100A